ZFIN Image: Liao et al., 2021, Fig. 4

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Figure Caption

Fig. 4

VD₃ enhances neutrophil recruitment to the wound site of amputated fish. a–c The caudal fin of 6 dpf Tg(mpx:gfp) zebrafish. Imaging and quantification of GFP⁺ neutrophils recruited to tail wound at 4 hpw (n = 4/group) (b). d qRT-PCR analysis of IL-8 transcripts in 6 dpf zebrafish larvae treated 0 nM, 10 nM, or 100 nM VD₃ for 4 days, with and without caudal fin amputation (n = 6 replicates/group, 6–15 larvae/replicate). Two-way ANOVA with Sidak test was used to test significance. For c, statistical comparisons were performed within each time point. Data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. dpf, days post fertilization; VD₃, vitamin D₃; WT, wild-type; hpw, hours post wounding; mpx, myeloperoxidase.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Innate Immun.