ZFIN Image: Liao et al., 2021, Fig. 3

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Fig. 3

Microbiota impact on granulopoiesis in zebrafish. a–c The gene expression of csf3b (a), mpx (b), and mmp9 (c) in CONVED or GF zebrafish larvae (6 dpf) treated 0 or 100 nM VD₃ for 4 days (results are combined from 2 independent experiments, n = 12 replicates/group, 10–20 larvae/replicate). d–f WT and cyp2r1 mutant zebrafish in 3 months were treated with antibiotics for 1 week, and the gene expression of csf3b (d) mpx (e), and mmp9 (f) transcripts in the gut was analyzed (n = 8 replicates/genotype). Two-way ANOVA with Sidak test was used to test significance. Data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. csf3b, colony-stimulating factor 3; mmp9, matrix metalloproteinase9; dpf, days post fertilization; VD₃, vitamin D3; WT, wild-type; CONVED, conventionalized; GF, germ-free; mpx, myeloperoxidase.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Innate Immun.