ZFIN Image: Al-Dahmani et al., 2022, Fig. 1

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Figure Caption

Fig. 1

Thiosulfate exposure does not affect viability or pronephros morphology in healthy zebrafish larvae. (A) Representative overview of the zebrafish appearance at 48 hpf with and without 10 mM thiosulfate. Zebrafish embryos exposed to 10 mM thiosulfate did not show any gross morphological changes compared to controls. (B) Representative visualization of the pronephros phenotype in Tg(wt1b:EGFP) embryos at 48 hpf with and without 10 mM thiosulfate. Thiosulfate did not have any visible effect on the pronephros morphology compared to controls. (C) Evaluation of the survival in zebrafish larvae exposed to thiosulfate for 6 days. Thiosulfate did not lead to any detrimental effects regarding survival in any of the studied concentrations (n = 15 for each condition). (D) Evaluation of the pronephric neck length in Tg(wt1b:EGFP) embryos at 48 hpf exposed to different thiosulfate concentrations. Thiosulfate did not significantly alter the pronephric neck length in healthy zebrafish larvae (at least 10 larvae per group were analyzed). Scale bars are 500 μm in (A) and 50 μm in (B). Statistics utilized one-way ANOVA + Sidak’s post-hoc (D); mean + SD reported; *p < 0.05.

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Phenotype details

Acknowledgments

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