Fig. 9

Fig. 9

(A) Diagram of expected Cre mediated inversion of rb1^on to ?off? orientation. (B, C) pH3 and HuC/D labeling of larval sectioned head tissue from 5 dpf transheterozygous rb1^on/stop (B ? B?) and Cre injected rb1^on/stop (C ? C?). (D, E) pH3 and Cre labeling of larval sectioned head tissue from 5 dpf transheterozygous rb1^on/stop (D ? D?) and neurod1-2A-Cre; rb1^on/stop (E ? E?). (F) Quantification of pH3-positive cells in control rb1^on/stop (n=3) and Cre rb1^on/stop (n=3) injected midbrain (**** p<0.0001) and retina (** p<0.01). (G) Genomic DNA qPCR quantification of rb1^onoriginal orientation DNA 5? and 3? junctions in control rb1^on/stop (n=3) and Cre injected rb1^on/stop (n=3). (H) Quantification of pH3-positive cells in control rb1^on/stop (n=3) and neurod1-2A-Cre; rb1^on/stop (n=3) midbrain (*** p<0.001) and retina (* p<0.05). (I) Genomic DNA qPCR quantification of rb1^on original orientation DNA 5? and 3? junctions in control rb1^on/stop (n=3) control and neurod1-2A-Cre; rb1^on/stop (n=3). Error bars represent mean ± s.e.m. with two-tailed t-test. Scale bars: 50 ?m (B - E), 20 ?m (B? ? E?).