FIGURE 2

FIGURE 2

Series of script-automated steps to obtain peak (systolic) RBC velocity from TrackMate data, demonstrated using data from zebrafish 28 of the 2 dpf data set. (A) Velocity sampling within spatial bins of controlled intervals in different vessel types: shown in bold line black boxes are the 60-µm intervals for (i) a region in the anterior DA/CA, (ii) a region in the posterior DA/CA and ventral-to-dorsal intervals of filtered data (see text) for aISVs at the (iii) anterior and (iv) posterior regions of the trunk vasculature. (B) Example of TrackMate particle tracking algorithm performed in the anterior DA/CA region (i) shown in (A). (C) Contiguous velocity against time curves and the application of peak scanning and outlier filtering to obtain the average peak (systolic) velocities in regions i, ii, iii and iv shown in (A). (D) The anterior-to-posterior distribution of average peak velocities in the DA/CA (left) and aISVs (right) with the values for the four locations (i–iv) highlighted in (A,C) shown in boxes.