Figure 5

Figure 5

(A) Growth of V. alginolyticus. Analysis of growth throughout the V. alginolyticus ΔpykF strain and WT:pykF overexpression strain at 24 h. (B) Analysis of growth throughout V. alginolyticus ΔpykF:pykF, ΔpykF:K52R, ΔpykF: K68R and ΔpykF:K317R complemented strains at 24 h. (C) Analysis of extracellular protease activity throughout the V. alginolyticus ΔpykF mutant strain and WT:pykF overexpression strain. Extracellular protease activity of V. alginolyticus WT was set as 1. (D) Analysis site-directed mutagenesis complemented strains on extracellular protease activity. Extracellular protease activity of V. alginolyticus ΔpykF:pykF was set as 1. Mean values and standard deviations were calculated based on three replicates (n = 3). Two-tailed P-values were determined by the t-test, and the significance level is 0.05. *p < 0.05.