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Figure 4
Top line, acetylation of PykF. SDS-PAGE and Western blot analysis were performed on purified PykF treated with AcP in different concentrations and incubation time. The same amount of protein was loaded. Anti-AcK, acetyl lysine antibody. Bottom line, pyruvate kinase activity was measured, and the enzyme activity of lane 1 sample was set as 1. Mean values and standard deviations were calculated based on three replicates (
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