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Fig. 5
CD146 promotes stemness in GBM cells. A Western blots showing increased expression of SOX2 and Oct-4 in CD146/GFP overexpressing GG16 neurospheres (NSP) and differentiated cells (Diff). B Representative images of GG16 control and GG16-CD146/GFP colonies in colony formation assay showing more dispersed cells in CD146 overexpressing cells (scale bars?=?200 ?m). C Quantified data of colony numbers in GG16 control compared with CD146/GFP overexpressing GG16 cells for 2000 cells plated. D, E Representative images (scale bars?=?400 ?m) and quantified data of GG16 control or CD146/GFP overexpression neurospheres in limiting dilution assays. F Western blots showing reduced SOX2 and Oct-4 expression in two different GSC23-CD146-ko cells compared to control serum-differentiated cells. G, H Representative images and quantified data of GSC23 control and two different GSC23-CD146-ko neurospheres in limiting dilution assay. CD146 loss reduces neurosphere formation potential (scale bars?=?200 ?m). I, J Representative images and quantified data of differentiated GSC23 control vs GSC23-CD146-ko in colony formation assays. CD146 loss reduces colony formation potential in differentiated GSC23 cells. *p?<?0.05; **p?<?0.01; ***p?<?0.001 by Student?s t test