Fig. 3

Clock gene dosage increases correlated transcriptional variability more than uncorrelated one

(A) Reduced copy of her genes likely results in decreased burst sizes in her1^b567/ci301;her7^b567/hu2526 mutants compared to her1^ci301;her7^hu2526 mutants.

(B) One of the chromosomes has mutant her1 and her7 genes whereas the other has a large deletion including the her1-her7 locus in her1^b567/ci301;her7^b567/hu2526 mutants. Red stars (?) mark point mutations causing premature stop codons.

(C) Expression pattern of her7 is disrupted in her1^b567/ci301;her7^b567/hu2526 embryos. Scale bar is 30 ?m.

(D) her1^b567/ci301;her7^b567/hu2526 embryos (n=12, N=2) have less her mRNA than double homozygous her1^ci301;her7^hu2526 embryos (n=17, N=2). The frequency histogram of total mutant her (her1+her7) RNA per cell is plotted in her1^ci301;her7^hu2526 (red), her1^b567/ci301;her7^b567/hu2526 mutant (blue) embryos.

(E and F) her1^b567/ci301;her7^b567/hu2526 embryos have both reduced correlated (E) and uncorrelated (F) transcriptional variability than her1^ci301;her7^hu2526. Error bars are two standard errors. n is the number of embryos; N is the number of independent experiments.