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Fig. 7 Efficacy of [2](PF6)2 in PC3Pro4 prostate cancer zebrafish ectopic model. (a) Schedule of tumour cells injection and treatment with [2](PF6)2 by water administration (WA). Around 300 PC3Pro4 cells were injected into Duct of Cuvier at 2 dpf. 0.5 ?M of [2](PF6)2 was added into water at 2.5, 3.5, 4.5, 5.5 dpf. At 3, 4, 5, 6 dpf, the compound was washed away, the embryos were irradiated with green light (21 mW cm?2, 90 min, 114 J cm?2, 520 nm). (b) Water administration of [2](PF6)2 into 6-well plates containing engrafted embryos. (c) The images of PC3Pro4 tumour burden at CHT site at 6 dpf. (d) The relative fluorescence intensity of PC3Pro4 tumour burden at 6 dpf. (e) The relative tumour area of PC3Pro4 tumour burden at 6 dpf schedule of tumour cells injections and treatment with 200 ?M of [2](PF6)2 by intravenous administration. (g) The injection site of intravenous administration (IV). (h) The images of PC3Pro4 tumour burden at CHT site at 6 dpf. (i) The relative fluorescence intensity of PC3Pro4 tumour burden at 6 dpf. (j) The relative tumour area of PC3Pro4 tumour burden at 6 dpf. Results are presented as means ± SD from three independent experiments.