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Fig. 1
The phenotype of embryos treated with PBCN. (A). Bright field images of the whole body and heart and fluorescence microscope images of the heart. (B,C). Under different concentrations of PCNB, 24, 48, 72 hpf, the survival rate and hatching rate of embryos. (D–F). A statistical graph of the embryo’s body length, heart rate, and atrioventricular distance after 72 h of treatment with different concentrations. SV-BA refers to the distance from the atrium to the ventricle, the red arrow in the figure points. (n = 3, Means ± SD. Scale bar: 500 μm/100 μm).
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