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Fig. 3
brsk2b-deficient zebrafish displayed developmental delay. (A) Lateral images of brsk2b+/+, brsk2b±, brsk2b–/– larvae at 1, 2, 3 dpf. Scale bars 500 μm. (B) The optic tectum (OT) and body length (L) were measured from lateral and dorsal images using ImageJ. The red line represents OT, and the blue lines represent body length. (C–E) The body lengths of brsk2b–/– larvae at 2 dpf (C), 3 dpf (D), 5 dpf (E) were significantly shorter than that of brsk2b+/+ larvae (n = 24 for each genotype, p = 0.0001, 0.0001, and 0.0043, respectively). (F) The values of OT of brsk2b–/– larvae at 5 dpf were smaller compared to that of brsk2b+/+ larvae (n = 24 for each genotype, p = 0.0001). (G) The ratio of OT/L in three genotypes didn’t show significant difference at 5 dpf (brsk2b+/+: brsk2b±: brsk2b–/– = 24: 23: 22, p = 0.385). Groups were compared using a one-way ANOVA followed by a Dunnett’s multiple comparisons test. Data are shown as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.