Fig. 7

Fig. 7

(A) Immunofluorescence (IF) showing Sox10-positive posterior lateral line nerve (pLLN) SCs (black/gray) at 55 hours post fertilization (hpf) in embryos treated with DMSO or 100 µM dexamethasone (Dex). Black dashed lines outline the pLLN. Sox10-positive pigment cells outside of the dashed lines (white asterisks) were not included in the analysis. (B) EdU incorporation assay showing sox10:nls-eos-positive, pLLN SCs (orange) pulsed with EdU (cyan) from 48 to 55 hpf in embryos treated with DMSO or 100 µM Dex. White dashed lines outline the pLLN. Magenta dashed lines outline neuromasts. (C) Scatter plot of the number of pLLN SCs at 55 hpf. Asterisks (*) indicate significant differences discovered with two-way ANOVA with Tukey’s post-hoc test using GraphPad Prism. Obelisk (†) indicates the significant difference discovered with Student’s t-test (GraphPad Prism) comparing WT DMSO and WT Dex alone (mean ± SEM: DMSO: WT: 12.0 ± 0.5, cd59^uva48: 18.1 ± 0.6; Dex: WT: 10.4 ± 0.4, cd59^uva48: 13.4 ± 0.6; two-way ANOVA p-values: WT DMSO vs. cd59^uva48 DMSO: p<0.0001, WT Dex vs. cd59^uva48 DMSO: p<0.0001, WT Dex vs. cd59^uva48 Dex: p=0.0014, cd59^uva48 DMSO vs. cd59^uva48 Dex: p<0.0001; t-test p-value (compared WT only): WT DMSO vs. WT Dex: p=0.0206; dot = 1 fish). (D) Scatter plot of the number of EdU-positive SCs along the pLLN at 55 hpf in embryos treated with DMSO or 100 µM Dex (mean ± SEM: DMSO: WT: 4.6 ± 0.85, cd59^uva48: 9.0 ± 0.68; Dex: WT: 2.7 ± 0.65, cd59^uva48: 3.5 ± 0.53; p-values: WT DMSO vs. WT Dex: p=0.0091, WT DMSO vs. cd59^uva48 DMSO: p=0.0266, WT Dex vs. cd59^uva48 DMSO: p<0.0001, cd59^uva48 DMSO vs. cd59^uva48 Dex: p<0.0001; dot = 1 fish). These data were compared with Student’s t-test using GraphPad Prism. All data were normalized to units per 100 µm. All images were acquired with confocal imaging. Scale bars: (A, B) 25 µm.