Life without rRNA acetylation (A) Simplified phylogenetic tree of Nematoda. Sequence contexts of the acetylated cytidine in representative species of each clade are indicated. (B) Sanger DNA sequencing of RT-PCR products obtained after borohydride treatments of total RNA extracted from C. elegans. (C) Ac4C content in total RNA extracted from adult C. elegans was visualized by Northern blotting-based assay using anti-Ac4C antibodies. Ethidium bromide (etBr) staining indicates the relative position of rRNA and tRNA species. (D) A ten-fold serial dilution of cell suspensions of the different yeast strains (as indicated on the side of the panels) was spotted on a YPD-agar plate and incubated for 48 h at low (19°C), regular (30°C) and high (37°C) temperatures. (E) Top-panels: WT and ΔsnR4ΔsnR45 yeast strains were grown at 30°C (left) or 37°C (right) in liquid YPD. Optical density at 600 nm (OD600) was measured every 30 min. Growth curves of individual biological samples (thin lines; n = 3 or 12 for 30°C and 37°C samples, respectively) and the mean of replicates (thick lines) are presented along with a confidence interval (gray area). Bottom-panels: WT and ΔsnR4ΔsnR45 yeast strains were spotted on a YPD-agar plate and incubated for 48 h at 30°C or 37°C. Cells were grown in duplicates with a 5-fold dilution between distinct concentrations.
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Nucleic Acids Res.