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Fig. 6
Wild?type and reversed Mrap2a/b differently affect the pharmacological profiles of zMc4r. (A?C) Dose?responsive stimulation of MC4R by ??MSH?induced cAMP production with different ratios of wild-type and reversed Mrap2a/b (A,B) or MRAP2 (C). (D?F) Dose?responsive inhibition of MC4R by antagonist SHU9119 with different ratios of wild-type and reversed Mrap2a/b (D,E) or MRAP2 (F). (G?I) The basal cAMP level caused by transfected MC4R, MRAP2 and RMRAP2 in the absence of an agonist. Data were analyzed by one?way ANOVA and shown as mean ± SEM of three replicates. ns (not significant difference), * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. 2a: Mrap2a, R2a: RMrap2a, 2b: Mrap2b, R2b: RMrap2b, M2: MRAP2, RM2: RMRAP2.