Fig. 1

Fig. 1

Disruption of the mouse Rbpr2 gene using the Cre/LoxP strategy and generation of the global Rbpr2 knockout (Rbpr2^−/−) mice. (A) An Rbpr2 targeting vector was designed to replace exon 7 with a neomycin cassette (neo^R) flanked by loxP sites. This resulted in the Rbpr2 neo^R cassette allele. The neo^R cassette was floxed out by mating Rbpr2^fl/wt mice with the Rosa^flp/flp mice. The Rbpr2^fl/fl mice were then mated with Actin-Cre+ mice to finally generate littermate controls and the global Rbpr2 knockout (annotated as Rbpr2^−/−) mice. (B) PCR-based genotyping for identifying the Rbpr2^fl/fl; Actin-Cre+ (Rbpr2^−/−) and littermate control mice. (C) A custom made RBPR2 antibody was generated and used to determine the systemic loss of RBPR2 protein expression in Rbpr2^−/− mice. Immunoblot of protein extracts of tissues from adult WT and Rbpr2^−/− mice. Pooled protein from n = 3 mice (per genotype) at 6–8 weeks of age. GAPDH antibody was used as the loading control. Int, intestine; WT, wild-type.