|
Fig. 5
Effects of PGF2α and PGE2 on mitochondrial function in zebrafish liver cells. Cells were incubated with or without PGF2α (10 μM) and PGE2 (10 μM) for 24 h. (a) Lipid droplets were stained with BODIPY (green), nuclei were stained with DAPI (blue), and mitochondria were stained with MitoTracker (red). (b) Average MitoTracker fluorescence per cell (n = 3). (c) Mitochondrial copy number (n = 4). (d) Relative transcript expression of lipid catabolism-related genes (n = 3). (e) Relative transcript expression of β-oxidation- and mitochondrial respiratory chain-related genes (n = 3). (f) ATP levels (n = 6). Statistical significance is denoted with asterisks as follows: * p < 0.05; ** p < 0.01; *** p < 0.001.