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Fig. 2
Effects of serum starvation on cyclooxygenase (COX) metabolism in zebrafish liver cells. (a) Schematic diagram of the COX pathway in arachidonic acid (ARA) metabolism. (b,c) Cells were incubated with either normal medium or serum-free medium for 24 h, and the relative transcript expression of COX metabolism-related genes (n = 3) and the concentration of ARA-derived COX metabolites in the cells and medium were measured (n = 5). (d,e) Cells were pre-treated with NC, ATG7, and ATGL siRNA and then serum-starved for 24 h, and the relative mRNA expression of COX metabolism-related genes (n = 3) and the concentration of PGF2α in cells (n = 6) were measured. Ptgs, prostaglandin-endoperoxide synthase; pgtes, prostaglandin E synthase; ptgds, prostaglandin D2 synthase; ptgis, prostaglandin I2 synthase; ptgfs, prostaglandin F synthase. Statistical significance is denoted with asterisks as follows: * p < 0.05; ** p < 0.01; *** p < 0.001; ns, not significant difference.