FIGURE 5

FIGURE 5

Early axon entry promotes early OPC migration to sensory nerves. (A) Images from a 24 h time-lapse movie starting at 48 hpf in Tg(sox10:gal4; uas:lifeact-gfp) zebrafish treated with Paclitaxel showing axon initiation and sensory-related OLC and DREZ contact earlier than typical axon entry. Green arrows represent migrating sensory-related OLC. (B) Still Images from a 24 h time-lapse movie starting at 48 hpf in intact and lesioned Tg(sox10:gal4;uas:lifeact-gfp);Tg(gfap:nsfb-mcherry) zebrafish also showing axon initiation and successful axon entry earlier than typical axon entry. White dashed line indicates lesion site. (C) Quantification of average time to axon entry in DMSO and Paclitaxel treated animals (p = 0.0081). (D) Quantification of the average time to sensory-related OLC migration post-axon entry in DMSO and Paclitaxel treated animals (p = 0.0338). (E,F) Paralleled quantifications of C-D in Lesioned and Non-lesioned animals (D = p < 0.0001, E = p = 0.0467). (G) Quantification of the average distance and time three OLCs migrated post-axon entry in DMSO and Paclitaxel treated animals and lesioned and non-lesioned animals. Orange arrowhead indicates start of timelapse at 48 hpf. Blue rectangles highlight the time of axon entry. Orange boxes indicate OLC-DREZ contact. Scale bar equals 10 μm (A,B). All images are orientated anterior to left, posterior to right, dorsal up and ventral down.