|
Fig. 4 Electra variants performance as fusion tags in live HeLa cells. (a) -terminal fusion of mTagBFP2, Electra1 and Electra2 with CytERM for OSER assay (one transfection each; see Supplementary Table S5). Representative images of healthy cells and cells with whorls are shown (403 nm excitation; 430–490 nm emission). The dynamic range of all images was adjusted independently to facilitate visualization. (b) From top left to right: COX8A-Electra1, paxillin-Electra1, β-actin-Electra1, keratin-Electra1, laminA-Electra1, α-tubulin-Electra1, H2B-Electra1, paxillin-Electra2, β-actin-Electra2, keratin-Electra2, laminA-Electra2, α-tubulin-Electra2, H2B-Electra2, α-tubulin-mTagBFP2 (imaging conditions: 403 nm excitation; 490 nm emission). The dynamic range of all images was adjusted independently to facilitate visualization.