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Fig. 7
fr.1 and saponarin inhibited the LPS-mediated interaction between TLR4 and MyD88 in THP-1 macrophages. Relative TLR4/MyD88 interactions in LPS-induced macrophages were determined by western blot of immunoprecipitation samples. (A) Western blot images of TLR4-co-immunoprecipitated (Co-IP) protein samples obtained from fr.1 or saponarin treated THP-1 macrophages. Co-IP proteins were detected using TLR4 or MyD88 antibodies. (B) Relative ratios of TLR4 interacting with MyD88 adaptor protein. Band intensities were measured by densitometry and normalized versus TLR4 band intensities. Results are presented as the means ± SDs of three different experiments. #p < .05 versus non-treated control macrophage group, and *p < .05 versus LPS-treated inflammatory macrophage group.
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