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Fig. 2 - supplement 1
(A) Immunofluorescence image of a 48 hours post fertilization (hpf) retina.Tg(h2a:mCh) labels all retinal nuclei (green), and collagen antibody marks collagen type IV (magenta). Bottom panel: Higher magnification inset of the retina show anti-collagen IV (gray). Red arrowhead: basement membrane. Scale bar: 50 μm. (B–B’) Stills from time-lapse of a retina at 50 hpf (B) and 60 hpf (B’). Tg(lhx-1:eGFP) labels HCs (green), Tg(βactin:maKate2-ras) marks cell membranes of all retinal cells (magenta). Scale bar: 50 µm. Right panels: Higher magnification insets of the outlined regions show cell membranes (gray). Dashed line: forming inner plexiform layer (IPL). Scale bar: 10 μm. (C) Confocal sections of Tg(βactin:lap2b-eGFP) labeling nuclear envelopes of all retinal cells (magenta), and Tg(βactin:maKate2-ras) labeling membranes of all retinal cells (green) at 65 hpf. Scale bar: 20 µm. (C’) Higher magnification insets of the outlined region showing nuclear envelopes (left panel) and cell membranes (right panel). Blue dashed line: region for intensity profile measurement in C”. Scale bar: 10 μm. (C’’) Line scan average fluorescent intensity profiles of βactin:lap2b-eGFP (magenta) and βactin:maKate2-ras (green) along the blue dashed line. See Figure 2—source data 1.
No collagen is found in the densely packed INL.