ZFIN Image: Yao et al., 2022, Fig. 5

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Figure Caption

Fig. 5

Conformational free energy of DrPCP3b versus pH

(A) The midpoint of the transition between native dimer (N₂) and the first dimeric folding intermediate (I₂) (●). The solid line represents a fit of the data to determine pKa, as described in the text. (B) Free energies for $Δ G_{1}^{H_{2} O}$ $Δ G_{1}^{H_{2} O}$ (●), $Δ G_{2}^{H_{2} O}$ $Δ G_{2}^{H_{2} O}$ (■), $Δ G_{3}^{H_{2} O}$ $Δ G_{3}^{H_{2} O}$ (♦) and $Δ G_{t o t a l}^{H_{2} O}$ $Δ G_{t o t a l}^{H_{2} O}$ (▲) versus pH. (C) Cooperativity indices, m₁ (●), m₂ (■), m₃ (♦) and m_total (▲) versus pH. Data for panels (B and C) were determined from global fits of the data shown in Supplementary Figure S2 and S3 to the models shown in (eqns 2 and 3), as described in the text. The error bars show the standard error obtained from the global fits.

Acknowledgments

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