Figure 2

Figure 2

nlrc3-like deficiency upregulates the expression of inflammatory genes during mycobacterial infection. (A) Quantitative RT-PCR showing expression of nlrc3-like in 3 dpf zebrafish embryos at 0, 1, 3, 5 and 7 days post infection with M. marinum:Wasabi.(B) Quantitative RT-PCR showing expression of multiple inflammatory genes at 1 dpi. The values shown represent an average of three independent biological experiments, 25 embryos per group in each experiment. (C)il-1β WISH shows significantly increased il-1β expression in nlrc3-like deficient mutants compared with their siblings at 1 dpi. Scale bar = 200 μm. (D) Co-staining of il-1β WISH (red) and anti-GFP antibody (green). White arrows point to co-staining signals of mfap4-GFP and il-1β, and white arrow heads point to the il-1β only signals. Scale bar = 50 μm **p ≤ 0.01; ***p ≤ 0.001; ns, not statistically significant (p>0.05).