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Fig. 3
Figure 3. Isolation and characterization of interGSCs derived from ovotestis. (A) Cell morphology of GSCs isolated from ovotestis at the indicated time after isolation. Red arrows show the grape-like GSCs. (B) Image of AP staining of interGSCs isolated from ovotestis. (C) Cell morphology showing the presence of two morphologically distinct cell types. (D) qRT-PCR results of cdh1 and stat3 in fGSCs, SSCs, and interGSCs, compared with whole ovary, testis, and ovotestis tissues. (E) Immunofluorescence images for day 7 interGSCs.
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