Fig. 4-supplement 2
(A) Diagram of the construct used to generate the UAS:dnSu(H) line. (B) Scheme of protocol used. (C–E) Trunk region of a Sox10:Kalt4;UAS:dnSu(H) embryo treated with tamoxifen from 11 to 24 hpf and immunostained for (C) RFP and (D) myc. (E) overlay. Dotted squares indicate enlargement. (F) Number of embryos expressing the UAS driven transcripts (myc+) after tamoxifen treatment from 11 hpf for different times (15′ n = 20, 30′ n = 27, 45′ n = 25, 1 hr n = 22, 3 hr n = 18, 5 hr n = 20, 24 hr n = 14, 48 hr n = 10).
UAS:dnSu(H) transgenic line.
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