Figure 1—figure supplement 1.

Figure 1—figure supplement 1.

(A) Sequence alignment of human SHP2 and zebrafish Shp2a polypeptides performed by ClustalW. The residue D61 is highlighted in red, while the identical amino acids are highlighted in black. The amino acid number is marked to the right of each row. (B) Schematic representation of the procedure used to establish the Shp2^D61G mutant line, as described in the Materials and methods section. (C) Representative images of the WISH staining for myl7, ahmc, and vhmc expression in the hearts of 3 days post fertilization (dpf) old Shp2^wt, Shp2^D61G/wt, and Shp2^D61G/D61G zebrafish embryos. Numbers in the pictures indicate the number of embryos with the phenotype represented in the image. Ejection fraction (D) and cardiac output (E) determined from the high-speed videos of the heart of 5 dpf old Shp2^wt, Shp2^D61G/wt, and Shp2^D61G/D61G zebrafish embryos. Measurements originate from at least three distinct experiments. Numbers on the bars depict the number of embryos. Error bars represent standard error of the mean (SEM).*p < 0.05; **p < 0.01, ***p < 0.001, NS, non-significant, ANOVA complemented by Tukey’s HSD.