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Figure 4

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Publication: Duclaux-Loras et al., 2022 - UNC45A deficiency causes microvillus inclusion disease-like phenotype by impairing myosin VB-dependent apical trafficking
All Figures: Figures for Duclaux-Loras et al., 2022

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Figure 4 MYO5B as a client of the HSP90-UNC45A chaperone complex.

(A) Volcano plot showing proteins enriched in the myc-tagged UNC45A WT immunoprecipitates over the myc-tagged EV control identified by mass spectrometry in 3 independent pull-down assays. The difference of the average of the logarithm of Label Free Quantification (LQF) intensities (WT vs. EV) is plotted against negative logarithmic P values of a 2-sided, 2-sample Welsh t test. The hyperbolic curve delimitates significantly enriched proteins from common hits. High LFQ hits of interest are indicated in black (HSP90 family), in purple (Myosin protein family), and in green (Actin protein family). (B) Accumulation of myosin VB aggregates (aggresomes) in UNC45A^KO cells treated with MG-132 (10 μM) overnight. Scale bars: 10 μm. n = 3. Nuclei are stained with Nucblue (blue).

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