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Fig. 6 Figure 6. Effects of GME on PHOSPHO-AKT pathway in colon cancer cells SW480. (A) SW480 cells were treated with the absence or presence of GME for 72?h and harvested to measure protein levels of AKT, P-AKT, P-GSK-3?, P-PDK1 and P-c-Raf by western blotting. We used the housekeeping protein GAPDH as a positive loading control in all experiments. (B) Relative density of phospho-AKT signalling pathway proteins. (C) Apoptosis-related markers such as poly ADP ribose polymerase (PARP) cleavage, caspases activities and Bcl-2 family of proteins were detected by western blotting in SW480 cells. (D) Apoptosis-related protein relative density.