Fig. 3

a Schematic of experimental setup to analyze locomotor behaviors of macrophage-rescued (+GH) and non-transgenic non-rescued (No GH) sibling zebrafish side by side. b Time plots showing the efficacy of the stable mpeg1-nlrc3l transgene to largely restore daytime locomotion in nlrc3l mutants from their reduced baseline level (shown in the non-rescued plot). The rescue transgene had no apparent effect on the heterozygous and wild-type siblings; no difference in locomotor behavior was found between siblings with and without the rescue transgene. c Scatter box-and-whisker plots showing no significant difference in daytime locomotion or activity in macrophage-rescued nlrc3l mutants compared with baseline wild-type siblings, while baseline nlrc3l mutants showed a significant reduction. n number of animals analyzed shown in parenthesis below each scatter plot. Three independent experiments were conducted to compare larvae derived from an nlrc3l heterozygous intercross with and without the macrophage rescue transgene on the same plate. Statistical significance was determined by two-way ANOVA test followed by multiple comparison tests. FDR-adjusted p-values are reported; ns, not significant as defined by p-values > 0.05. All pairwise brackets not shown are ns, not significant. Res, individuals carrying the rescue transgene.