The effects of LPS on cell viability and apoptosis of ZFL cells. (A) The cell survival rates of ZFL cells treated with lower concentrations of LPS for 24 h. (B) The cell survival rates of ZFL cell treated with higher concentrations of LPS for 24 h. (C) The intracellular ROS of ZFL cells treated with 100 μg/ml LPS for 24 h. (D) The cell survival rates and (E) cell apoptotic rates of ZFL cells co-treated with LPS and tempo for 24 h. (F) The representative images of cell apoptosis of ZFL cells co-treated with LPS and tempo for 24 h. The activities of (G) caspase-8 and (H) caspase-3 in ZFL cells co-treated with DHA and tempo for 24 h. The values are means ± SEMs (n = 3–8 biological replicates). The mean values without a common letter are significantly different, p < 0.05. *p < 0.05, **p < 0.01.
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