Figure 6

Figure 6

The effects of antioxidant, 4-hydroxy-Tempo (Tempo), on DHA cytotoxicity in ZFL cells. (A) The cell survival rates of ZFL cells treated with increasing concentrations of DHA for 24 h. (B) The cell survival rates and (C) the cell apoptotic rates of ZFL cells co-treated with DHA and tempo for 24 h. (D) The representative images of cell apoptosis of ZFL cells co-treated with DHA and tempo for 24 h. (E) The MDA levels in ZFL cells co-treated with DHA and tempo for 24 h. The activities of (F) caspase-9 and (G) caspase-3 in ZFL cells co-treated with DHA and tempo for 24 h. Values are means ± SEMs (n = 3 ~ 8 biological replicates). The mean values without a common letter are significantly different, p < 0.05. **p < 0.01.