Fig. 2

Figure 2. ZIKV infection of one-cell stage embryo changes locomotor activity behavior. The 96 hpf larvae infected with ZIKV^BR at 0 hpf or control group were distributed in a 96-well plate (1 larva/well), and the locomotor activity was analyzed in Zebrabox system. The larvae were exposed to an incubation period of 30 min in the light (lux: 12%) followed by the testing phase consisting of a dark stimulus (lux: 0%) for 1 s, alternated by 29 s in the light (lux: 12%) for 2.5 min to induce visual stimulation and the startle response. The total distance moved (mm) by each group during the testing period (A) and the average velocity of each group in mm/s are shown in (B). Graphical representation of larvae tracking during the testing phase (C). The lines represent the distance moved by the larvae at each second during the total experiment period with highlights of testing phase (D). Each bar represents the mean ± SEM. * p < 0.05 compared with the negative control group.