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Blackwell et al., 2022 - Hnrnpul1 controls transcription, splicing, and modulates skeletal and limb development in vivo

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Fig. 1.

Mutation of human HNRNPUL1 and zebrafish hnrnpul1 and hnrnpul1l. a) Schematic showing domains of human HNRNPUL1 and zebrafish Hnrnpul1 and Hnrnpul1l proteins. The mutation location in human HNRNPUL1 and equivalent sequence in zebrafish is marked by *. b) Comparisons of the locations of human HNRNPUL1 wild type, human mutant, and zebrafish hnrnpul1^ca53 and ^ca54 protein sequences showing frameshifted sequence (lighter font) following the mutation. Zebrafish hnrnpul1l wild type is compared to zebrafish hnrnpul1l in the exon 11 region where splicing to exon 13 creates a frameshift and stop codon after the new splice junction. c–f) WISH staining for hnrnpul1 (c, d) and hnrnpul1l (e, f), at 24 hpf reveals expression that is not spatially restricted in wild types (c, e), but is reduced in maternal zygotic (MZ) hnrnpul1/1l mutants (d, f). g) Quantification of SL normalized to the mean wild-type SL of 16 dpf larvae. Wild type n= 115, hnrnpul1/1l MZ mutant n= 127. ****P < 0.0001, determined by Student’s t-test. Scale bars = 200 µm.

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This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ G3 (Bethesda)