Figure 1

Figure 1

Identification of biliverdin as a potential NR2E3^LBD ligand. (A) A schematic representation of the method. Small molecules extracted from rabbit retina were incubated with recombinant MBP-NR2E3^LBD expressed and purified from E. coli. Protein-bound small molecules were isolated by a gel filtration chromatography and identified by mass spectrometry. (B) Mass spectra (ESI, positive ions) of metabolites co-purified with MBP-NR2E3^LBD, ones eluted in the gel filtration fraction without any protein, and commercial biliverdin. (C) A schematic representation of biliverdin and its metabolism (HO-1/2: heme oxygenases 1 and 2, BVRs: biliverdin reductases).