Figure 5.

Figure 5.

The temperature-dependent increase in mEPC events was greatly impaired by the inhibition of transmitter refilling. A, Traces of mEPC recorded at 25°C or 30°C from Baf A1-treated or Baf A1-untreated (control) muscles. B, Temperature dependency of mEPC frequency in control (n = 11 cells from 11 fish) and Baf A1-treated (n = 9 cells from 9 fish) conditions. In both conditions, temperature rise resulted in a significant increase in the mEPC frequency (**p < 0.01, paired t test). C, Fold change in mEPC frequency resulting from temperature rise. Baf A1 treatment significantly decreased the temperature dependency of mEPC frequency (*p < 0.05, unpaired t test). D, Temperature dependency of mEPC amplitude analyzed from the same datasets as in B. Temperature rise significantly reduced mEPC amplitude only in the Baf A1 condition (**p <0.01, paired t test). Right panels, Histograms of mEPC amplitudes recorded from representative cells. Error bars indicate ±SEM.