IMAGE

Fig. 2

ID

ZDB-IMAGE-220430-5

Antibodies

Ab7-h2afx

Publication

Annus et al., 2022 - Bloom syndrome helicase contributes to germ line development and longevity in zebrafish

All Figures

Figures for Annus et al., 2022

Image

Figure Caption

Fig. 2 Loss of Blm function does not increase sensitivity to DNA damage, however severely reduces lifespan in zebrafish.

A, A′ Anti-γ-H2AX labeling in the head and trunk of 3 dpf untreated wild-type (A) and blm^−/− embryos (A′) (Scale bar: 500 μm). B, B′ Anti-γ-H2AX labeling in the head and trunk of 3 dpf wild-type (B) and blm^−/− (B′) animals after treatment with the DNA interstrand cross-linking agent diepoxybutane (DEB) (Scale bar: 500 μm). C, D γ-H2AX-positive foci in the head and trunk of different blm genotypes under control circumstances (C) and after DEB treatment (D) (ns not significant). E Phenotypes observed after gamma irradiation: Class 1—wild-type; Class 2—mild necrosis in the tectum and smaller eyes; Class 3—heavy necrosis all over the body, heart edema. Untreated controls were all Class 1. F, G The distribution of phenotype severity following gamma irradiation (F) and DEB treatment (G) (ns not significant). H Survival probability graphs of different blm genotypes. (Pairwise p values were calculated with the log-rank test, using Benjamini & Hochberg adjustment. dpf days post-fertilization).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Dis.