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FIGURE 3 A representative graph of the light dark challenge assay: After an initial acclimation period of 10 min in the illuminated chamber (A), video tracking was initiated for another 10 min (AV) before the larvae were subjected to a dark challenge phase of 5 min (D1) and another light challenge phase of 10 min (L1). This challenge chain of dark and light phases was repeated three times (D2–L3), thus resulting in six alternating dark and light challenge phases. Swimming activity as distance moved (D, mm) typically shows intermediate levels during acclimation, elevated levels during the dark challenge and reduced levels during light challenge levels (mean ± SD, N = 152).