RecQ homologs in the zebrafish genome and their expression.
A Phylogenetic relationships of the five RecQ homologs that can be identified in the zebrafish genome based on similarities between the helicase ATPase and helicase C-terminal domains. (M. hungatei Hel308 DNA helicase was used as an external reference for eukaryotic RecQs). B, C The expression of genes related to double-strand DNA breaks during zebrafish development. Fragments per kilobase of exon model per million reads mapped (FPKM) and transcripts per kilobase million (TPM) values of the different genes at given stages as shown by the datasets in the respective papers (White et al., 2017; Winata et al., 2018). D Spatial distribution of blm RNA during early stages of development detected by whole-mount in situ hybridization. The number of embryos showing the expression pattern is indicated in the upper right corner. (All lateral views; for shield and 16 somite stages embryos ventral is to the left and dorsal to the right, while for 1 and 2 dpf embryos anterior is to the left and posterior is to the right, respectively. Scale bars: 150 μm.). E Schematic domain structure of zebrafish Blm and the position of the p.Thr101Leufs*4 mutation. (RQC RecQ-C-terminal domain, HRDC helicase and RNaseD C-terminal.). F Sanger sequencing shows the presence of the c.301_304delACAAinsTT allele (elu15) in exon 4 of a blm−/− embryo.
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