Transcriptional regulatory activity of the miR-29b-1 and miR-29b-2 gene promoters. (A) The location of the CpG sites in the promoter region of the miR-29b-1 gene (−873 bp to +158 bp). (B) The distribution of the CpG sites and three pairs of primers designed for bisulfite sequencing PCR in the promoter region of the miR-29b-1 gene. (C) The location of the CpG sites in the promoter region of the miR-29b-2 gene (−1495 bp to −1077 bp). (D) The distribution of the CpG sites and the primers designed for bisulfite sequencing PCR in the promoter region of the miR-29b-2 gene. (E) The effect of the −873 bp to +158 bp region on the regulation of miR-29b-1 gene promoter transcriptional activity. (F) The effect of the −1407 bp to -1173 bp region on the regulation of miR-29b-2 gene promoter transcriptional activity (***p < 0.001).
Acknowledgments
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