ZFIN Image: Xue et al., 2022, Figure 4<tag0:x xml:space='preserve'>. </tag0:x>

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Figure Caption

Figure 4.

Oxidative stress induced by CSE caused abnormal FN assembly. (a) Immunofluorescence staining for FN and phalloidin in CSE-exposed HUVECs treated with or without NAC (the arrows indicate FN fibrils). (b) Quantification of the counts of FN fibrils in HUVECs. (c) Immunostaining for FN matrix among vascular endothelial cells in CSE-exposed zebrafish embryos treated with or without NAC (5 mM) for 48 h (the arrows indicate FN fibrils among the flk⁺ cells). (d) Quantitative analysis of the FN matrix among vascular endothelial cells (n = 9). (e) Quantitative polymerase chain reaction (qPCR) for the expression levels of STAT3 mRNA in CSE-exposed HUVECs treated with or without NAC (1 mM) for 24 h. Data presented are the mean ± s.e.m. Unpaired Student's t-test, ^#p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05.

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