Figure 1

Figure 1. GFP retinal half-mounts showing the resolve of the lesion with the remaining scar; arrows point to the optic nerve for easier orientation: (i) (A) Uninjured (0 dpi) retinal half-mount with no lesion. (B,C) 1 and 2 dpi: lesion area is represented with a *, corresponding to a darker shaded area within the half-mount. (D) 3 dpi: the original circular lesion (*) has changed and there is a brighter margin surrounding the left side of the lesion (circled). (E) 7 dpi: reduced size of the lesion (*) and increased fluorescence within the margin. (F) 10 dpi: closing of the lesion with increased intensity of fluorescence, CMZ has been disrupted (circled). (G) 30 dpi: the lesion has resolved, and only reduced margin is visible near the CMZ (circled). (H) 60 dpi: the margin shows gathering of the tissue as the retina reaches the defect area, resulting in a fold at the site (circled). Each half-mount represents a separate animal. Scale bars represent 250 µm. (ii) Surrounding MG fluorescence intensity. The response to cryo-injury was quantified by calculating the percentages of the lesion area relative to the total area of the retinal half-mount. Linear regression was performed for (1) lesion size throughout 1–7 dpi and (2) MG proliferation between 10 and 60 dpi. A strong association for lesion size reducing from 1 to 10 dpi (p value = 0.00626). MG proliferation was notably increased from 10 dpi (p-value 0.0508, ns). Time points 1 dpi, 2 dpi and 3 dpi n = 5 independent animals and time points uninjured, 7 dpi, 10 dpi, 30 dpi and 60 dpi contain n = 6 independent animals.