ZFIN Image: Sheng et al., 2022, Figure 4

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Figure 4

Figure 4. Deficiency of sema6D caused aberrant vascular networks. (A) Confocal imaging analysis of ISVs in the control and sema6D-MO embryos at 48 and 72 hpf. (B) The statistical analysis of the ratio of vessels that span different segments in the control and sema6D morphants at 48 and 72 hpf (n = 7). About 9–12 ISVs were used for statistics per zebrafish. Mann-Whitney test, P = 0.0012.Values with ** above the bars are significantly different (P < 0.01).(C) The statistical analysis of the ratio of shorter vessels in the control and sema6D morphants at 48 and 72 hpf (n = 7). About 9–12 ISVs were used for statistics per zebrafish. Mann-Whitney test, 48 hpf: P = 0.0006; 72 hpf: P = 0.0012. Values with ** and *** above the bars are significantly different (P < 0.01 and P < 0.001, respectively).

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Acknowledgments

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