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Figure 7—figure supplement 4.

ID: ZDB-IMAGE-220426-66
Publication: Cornean et al., 2022 - Precise in vivo functional analysis of DNA variants with base editing using ACEofBASEs target prediction
All Figures: Figures for Cornean et al., 2022

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Figure 7—figure supplement 4. Phenotypic categorization of cytosine base edited embryos in medaka in F0.

(a) Microinjection of evoBE4max with the respective sgRNA for dapk3 (n = 74), ube2b (n = 91), usp44 (n = 79), and ptpn11 (n = 85) results in an increase in cardiovascular-associated phenotypes (‘cardiac’) compared to oca2 control editing (n = 62). (b) Exemplary phenotypes of 4 dpf embryos for the categories given in (a) (ventral view) with highlighting of the ventricle (V) and atrium (A). Here, embryos with morphological abnormalities or looping defects are shown in the ‘cardiac’ category. Quantification of base editing efficiencies (supplement 2) for the individual embryos is shown for the oca2 control (here: sequencing results for the four candidate loci given as range) and the ‘cardiac’ examples of the respective experiment. Scale bar = 100 µm. (c) Sub-categorization of the ‘cardiac’ and ‘global’ phenotypic groups shown as percentage of the sum of the two categories. dpf = days post fertilization.

Acknowledgments

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