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Figure 2—figure supplement 2.

ID: ZDB-IMAGE-220426-43
Publication: Cornean et al., 2022 - Precise in vivo functional analysis of DNA variants with base editing using ACEofBASEs target prediction
All Figures: Figures for Cornean et al., 2022

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Figure Caption

Figure 2—figure supplement 2. ABE8e efficiently introduces A-to-G mediated missense mutations in medaka and zebrafish.

(a–b) EditR quantification of Sanger sequencing reads for pooled oca2 ABE8e experiments (Q256, T306, Q333) from pools of five medaka embryos per data point, summarizes editing efficiencies for Q256 (a) and T306 (b) loci. For mean values see Supplementary file 2. To highlight the dinucleotide context, the nucleotide preceding the target A is shown by red (A), green (T), blue (C) and yellow (G) squares below the respective A. (c) Wider window of exemplary Sanger sequencing reads for individual, base edited embryos, from each experimental condition following ABE8e experiments at the zebrafish oca2-L293 locus.

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