FIGURE 2

FIGURE 2

Zebrafish retina undergoes neurodegeneration with ageing, independently of telomerase. The central retina immunolabelled with HuC/D and PKC (amacrine in magenta and bipolar cells in green, respectively), in both WT and tert^−/−, in (a) young (5 months) and (b) old adults (>30 months in WT and 12 months in tert^−/−). Scale bars: 20 μm. (c–f) Quantifications of the number of HuC/D‐positive neurons per area (10,000 μm²) (c) in the overall retina, and (d) in the GCL (ganglion cells) and (e) INL (amacrine cells). (f) Quantifications of the number of PKC‐positive neurons cells per area (10,000 μm²) in the INL (bipolar cells). Error bars represent the SEM. N = 3–7. (g–i) The central retina immunolabelled with (g) Ribeye A (BC synaptic terminals, in green), (h) 1D4 (outer segments of long double‐cones, in green), and (i) ZO1 (outer limiting membrane, in red), in both WT and tert^−/−, in young (5 months) and old adults (>30 months in WT and 12 months in tert^−/−). Scale bars: 20 μm. (g’–i’) Quantification of the percentage of fish presenting defects in (g’) Ribeye A (phenotype observed as disorganised synaptic terminal layering in the IPL), (h’) 1D4 (short and/or misaligned long double‐cone outer segments), and (i’) ZO1 (broken outer limiting membrane). Error bars represent the SEM. (g’) N = 4–9, (h’, i’) N = 3–6