Figure 2

Figure 2 Precocious vertebral bone development in hmx1^mut150 zebrafish embryos: Cartilage structures stained with alcian blue in wildtype and hmx1^mut150 embryos (a,b) at 5 dpf; the developing cranial cartilage structures develop properly in hmx1-mutant embryos. Cranial bone structures stained with alizarin red in wildtype and hmx1^mut150 at 5 and 7 dpf (c,f). Wildtype and hmx1^mut150 cranial bones developed regularly at 5 dpf (c,d), while at 7 dpf early mineralization of the vertebrae was detected in hmx1^mut150, similarly to hmx1^mut10 embryos (e,f). Bar, (a–c) 250 μm; (e–f) 500 μm.