Figure 3

Figure 3 Figure 3. rGO developmental toxicity. rGO exposures of embryos (n = 32) revealed that the 400 nm × 400 nm rGO did not cause significant mortality at any of the concentrations investigated (0 μg/mL, 2.32 μg/mL, 5 μg/mL, 10.7 μg/mL, 23.2 μg/mL, 50 μg/mL) (p > 0.05). The 2 μm × 2 μm rGO exposures caused mortality and malformations starting at 10.7 μg/mL. MO24 = 24 hpf mortality; MORT = 120 hpf mortality; any.effect = physical malformations, including mortality at 120 hpf. Outcomes significantly above the binomial threshold (Student’s t-test; α = 0.05) are indicated in red on the dot plot.