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Fig. 2.
svep1 loss-of-function sensitises angiogenic remodelling to reduced blood flow. (A) Bilateral quantifications of the percentage of gaps in the DLAV at 48?hpf in embryos injected with MO-CTL (5?ng) and MO-svep1 (5?ng) and treated with 0× (n=14 MO-CTL, n=20 MO-svep1), 0.5× (n=16 MO-CTL, n=24 MO-svep1), 1× (n=22 MO-CTL, n=27 MO-svep1) or 2× (n=21 MO-CTL, n=27 MO-svep1) from 30 to 48?hpf (N=3). (B) Bilateral quantifications of the percentage of lumenised segments in the DLAV at 48?hpf in embryos injected with MO-CTL (5?ng) and MO-svep1 (5?ng) and treated with 0× (n=14 MO-CTL, n=20 MO-svep1), 0.5× (n=16 MO-CTL, n=24 MO-svep1), 1× (n=22 MO-CTL, n=27 MO-svep1) or 2× (n=21 MO-CTL, n=27 MO-svep1) from 30 to 48?hpf (N=3). (C) Bilateral quantifications of the percentage of gaps in the DLAV at 48?hpf in embryos injected with MO-CTL (5?ng) (n=13), MO-gata1 (8?ng) (n=12), MO-svep1 (5?ng) (n=16) and MO-gata1 (8?ng)/MO-svep1 (5?ng) (n=25) (N=3). (D) Bilateral quantifications of the percentage of lumenised segments in the DLAV at 48?hpf in embryos injected with MO-CTL (5?ng) (n=13), MO-gata1 (8?ng) (n=12), MO-svep1 (5?ng) (n=16) and MO-gata1 (8?ng)/MO-svep1 (5?ng) (n=25) (N=3). (E) Bilateral quantifications of the percentage of gaps in the DLAV at 48?hpf in embryos injected with MO-CTL (5?ng) (n=11), MO-tnnt2a (4?ng) (n=12), MO-svep1 (5?ng) (n=12) and MO-tnnt2a (4?ng)/MO-svep1 (5?ng) (n=21) (N=3). (F) Bilateral quantifications of the percentage of lumenised segments in the DLAV at 48?hpf in embryos injected with MO-CTL (5?ng) (n=11), MO-tnnt2a (8?ng) (n=12), MO-svep1 (5?ng) (n=12) and MO-tnnt2a (4?ng)/MO-svep1 (5?ng) (n=21) (N=3). (G) Maximum intensity projection of dorsal view of the DLAV from representative embryos injected with MO-CTL (5?ng) (n=11), MO-tnnt2a (8?ng) (n=12), MO-svep1 (5?ng) (n=12) and MO-tnnt2a (4?ng)/MO-svep1 (5?ng) (n=21) embryos at 48?hpf. Red asterisks indicate gaps. (H) Bright-field images of klf2a in situ hybridisation of 48?hpf embryos injected with MO-CTL or MO-svep1 and treated with 0× or 2× tricaine from 30 to 48?hpf. Data are meanąs.d. Mann?Whitney test. Scale bars: 10??m (G); 50??m (H).