Survival rates of zebrafish larvae challenged with NNV through different infection routes and NNV replication. (A) Schematic representation of the different infection routes used to determine the susceptibility of zebrafish larvae to NNV. (B) Kaplan–Meier survival curves of NNV-infected and uninfected larvae in 3- and 14-dpf larvae. Mortality was registered during the next 10 dpi. (C) Quantification of NNV capsid protein gene expression in 3-dpf larvae infected via the brain or intramuscularly at different sampling points through qPCR; data are presented as the mean ± SEM of biological replicates. Statistically significant differences are displayed as follows: ***, 0.0001 > p value > 0.001.
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